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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Curr Protoc Immunol. 2013 Oct 1;102:12.14.1–12.14.30. doi: 10.1002/0471142735.im1214s102

Figure 1.

Figure 1

Measuring T-cell Phenotype in macaques. A Gating strategy: Mononuclear cells are first gated on single cells, then live lymphocytes or cells that are negative for the Amine dye are gated, followed by gating CD3+ T cells and then CD8+(green) and CD4+ (blue) subsets. B. Four CD4 T cell populations can be identified in the blood or rectum in macaques using CD28, CD95 and CCR7. First naïve (CD28+CD95−) cells and memory (CD95+CD28+/−) cells are gated. The memory population can then be differentiated by CD28 and CCR7 into central memory cells (TCM:CD28+CCR7+), transitional memory (TRN M: CD28+CCR7−) and effector/effector memory cells (TEM: CD28−CCR7−)cells. C Mononuclear cells isolated from rectal biopsies obtained from a vaccinated SIV infected animal, 10 days post SIV infection are gated using the strategy shown in A, CD8 memory T cells are then gated (CD95+CD28+/−) and the frequency of SIV Gag specific memory cells to the CM9 epitope are determined using an MHC Pentamer.