Table 3.

Primer sequences and PCR conditions used for realtime PCR

DOI: http://dx.doi.org/10.7554/eLife.01603.017

Primer	Primer-Sequence	Annealing temperature (°C)
r-ANP forward	5′-GCCGGTAGAAGATGAGGTCA-3′	60
r-ANP reverse	5′-GGGCTCCAATCCTGTCAATC-3′	60
r-aMHC forward	5′-GGAAGAGCGAGCGGCGCATCAAGG-3′	55
r-aMHC reverse	5′-GTCTGCTGGAGAGGTTATTCTCG-3′	55
r-bMHC forward	5′-CAAGTTCCGCAAGGTGC-3′	55
r-bMHC reverse	5′-AAATTGCTTTATTGTGTTTCT-3′	55
r-b2m forward	5′-CATGGCTCGCTCGGTGACC-3′	60
r-b2m reverse	5′-AATGTGAGGCGGGTGGAACTG-3′	60