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. Author manuscript; available in PMC: 2014 Feb 11.
Published in final edited form as: J Mol Biol. 2012 May 9;421(1):112–124. doi: 10.1016/j.jmb.2012.05.006

Fig. 4.

Fig. 4

Fab12 and Fab1068 bind TLR3ecd in the TLR3ecd:dsRNA SU. (a) Model of Fab1068 and Fab12 binding to a single SU. Fab binding does not inhibit dsRNA binding, TLR3 dimerization, or membrane association of TLR3. (b) SEC of various combinations of TLR3ecd (T), Fab1068 (F), and 49 bp dsRNA (R) indicate the presence of the R+T+F ternary complex. The R+T+F peak was collected and concentrated for EM. (c) SEC of various combinations of TLR3ecd (T), Fab12 (F12), and 49 bp dsRNA (R) indicate formation of R+T+F12 ternary complex. (d) EM 2D class averages of the ternary dsRNA:TLR3ecd:Fab1068 complex. 2D projections (middle panel) of a composite model complex (left panel) and selected class averages showing molecular complexes of dsRNA:2TLR3ecd:2Fab and dsRNA:2TLR3ecd:1Fab (right panel).