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. Author manuscript; available in PMC: 2015 Feb 4.
Published in final edited form as: Cell Metab. 2014 Feb 4;19(2):285–292. doi: 10.1016/j.cmet.2013.11.022

Figure 1. Hypoxia down-regulates glutamine oxidation, see also figure S1.

Figure 1

Panel A. Mitochondrial oxygen consumption (OCR) in control and DMOG treated SAS cells (500 μM 16h). OCR was measured in basal DMEM without serum, containing only 5 mM glucose, or only 2 mM glutamine or both as indicated.

Panel B. OCR in VHL-deficient RCC4 (constitutively active HIF1) and RCC4 cells with VHL reintroduced, treated as in A.

Panel C. OCR in empty vector SAS or ShHIF1α SAS treated as in A.

Panel D. Pyruvate dehydrogenase J(PDH) enzyme activity in SAS, RCC4 and RCC4-VHL cells in control conditions, or after 16h of 0.5% oxygen, or 500 μM DMOG.

Panel E. αKGDH enzyme activity in SAS, RCC4 and RCC4-VHL cells, treated as in D.

Panel F PDH activity in ShHIF1α SAS cells, treated as in D.

Panel F αKGDH activity in ShHIF1a SAS cells, treated as in D.

Data are represented 3 independent replicates ± S.D.