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. Author manuscript; available in PMC: 2014 Nov 13.
Published in final edited form as: Cell Host Microbe. 2013 Nov 13;14(5):571–581. doi: 10.1016/j.chom.2013.10.009

Figure 3. Metatranscriptomic reads annotated as flagellin are phylogenetically diverse.

Figure 3

Taxonomic assignments of flagellin gene transcripts are shown to the species level, with abundance of reads corresponding to the key at bottom. Hierarchical classification and the Subsystems database in MG-RAST was used to annotate function. We isolated reads with an annotation of flagellin and assigned taxonomy using BLASTX with default arguments. Note that Bacteroides pectinophilus is a Firmicute based on 16S rRNA gene sequence analysis but misclassified in the phylum Bacteroidetes in the MG-RAST database. Relates to Figure S3, which shows stimulation of TLR5 by flagellins from different species, and to Table S2.