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. Author manuscript; available in PMC: 2014 Nov 5.
Published in final edited form as: Curr Protoc Microbiol. 2013 Nov 5;31:9B.2.1–9B.2.7. doi: 10.1002/9780471729259.mc09b02s31

Figure 1.

Figure 1

Growth curves of L. monocytogenes 10403s. A freshly isolated colony of L. monocytogenes 10403s was used to inoculate 4 ml BHI broth which was incubated statically at 30 °C overnight (~16 h). The OD600 of the culture was measured the following day and is denoted by “X”. The overnight growth was back-diluted into fresh BHI to give an OD600 of 0.05 in a total volume of 25 ml in a 125 ml flask. Cultures were incubated with orbital shaking at 200 rpm at either 30 °C (black symbols) or 37 °C (white symbols), and OD600 measurements were taken hourly.