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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Dec 5;34(2):331–337. doi: 10.1161/ATVBAHA.113.302586

Figure 2. Epsin deficiency promotes VEGF-dependent in vitro angiogenesis.

Figure 2

A, Quantification of EdU incorporation into WT or DKO MECs after labeling in the absence or presence of VEGF-A (50 ng/mL) for 24 h. B, WT or DKO MEC monolayers were subjected to a scratch assay in the absence or presence of VEGF-A (50 ng/ml) for 12 h. C, Wound distance in B at 12 h was quantified using NIH ImageJ software. D, WT or DKO MECs were subjected to a tube formation assay by culturing on matrigel for 16 h in the absence or presence of VEGF-A (50 ng/ml). E, Tube formation in D at 16 h was quantified as in C. F, Plasma membrane fractions from WT or DKO MECs stimulated with 50 ng/mL VEGF-A for 0, 5 and 15 min were immunoblotted using the indicated antibodies. A, C and E, error bars indicate the mean ± s.e.m. n > 5, *p < 0.05. Scale bars: B and D, 50 μm.