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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Dec 5;34(2):331–337. doi: 10.1161/ATVBAHA.113.302586

Figure 4. Genetic reduction of VEGFR2 expression rescues in vitro angiogenesis caused by epsin deletion.

Figure 4

A, WT, DKO or DKO-Flkfl/+ MECs were stimulated with 50 ng/mL VEGF-A then whole cell lysates were immunoblotted using the indicated antibodies. B, WT, DKO or DKO-Flkfl/+ MEC monolayers were subjected to a scratch assay in the absence or presence of VEGF-A (50 ng/ml) for 12 h. C, Wound distance in B at 12 h was quantified using NIH ImageJ software. Error bars indicate the mean ± s.e.m. n > 5, *p < 0.05. Scale bars: B, 50 μm.