A, WT, DKO or DKO-Flkfl/+ MECs were stimulated with 50 ng/mL VEGF-A then whole cell lysates were immunoblotted using the indicated antibodies. B, WT, DKO or DKO-Flkfl/+ MEC monolayers were subjected to a scratch assay in the absence or presence of VEGF-A (50 ng/ml) for 12 h. C, Wound distance in B at 12 h was quantified using NIH ImageJ software. Error bars indicate the mean ± s.e.m. n > 5, *p < 0.05. Scale bars: B, 50 μm.