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. Author manuscript; available in PMC: 2014 Feb 11.
Published in final edited form as: NMR Biomed. 2011 Aug 23;25(2):379–388. doi: 10.1002/nbm.1764

Figure 3. Effect of perifosine on ChoK.

Figure 3

(A) Choline kinase activity: 1H MR spectra of MCF-7 cell lysate at the beginning and at the end of ChoK activity assay measurement. The conversion of choline to PCho was monitored throughout the reaction time period. Representative plot of PCho biosynthesis versus time from control ( ◇ ) and treated (■) cells. The data was fit to a straight-line to obtain the rates of PC synthesis. (B) Choline kinase expression: mRNA levels of CHKα and CHKβ in treated cells expressed as % of the control from Q-PCR analysis.