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. Author manuscript; available in PMC: 2014 Feb 11.
Published in final edited form as: NMR Biomed. 2011 Aug 23;25(2):379–388. doi: 10.1002/nbm.1764

Figure 4. Effect of perifosine on CT.

Figure 4

(A) Representative 13C spectra of lipid fraction showing the effect of treatment on de novo synthesis of PtdCho. (B) 31P spectra of MCF-7 cell lysate at the beginning and the end of CT activity assay measurement. The concentration of reaction product, CDP-Choline, was plotted against time, and the synthesis rate was determined from the slope of the straight-line fit. (C) Representative plot of CDP-Choline biosynthesis versus time in control cell lysate ( ◆ ) and perifosine-treated cell lysate ( ◇ ). (D) Representative plot of CDP-Choline biosynthesis versus time from extracted cell lysate plus EtOH ( ■ ) and extracted cell lysate plus 30 μM perifosine ( □ ).