Table 1.

Summary of changes in metabolite levels in cancer cells with 30μM perifosine

Metabolite (fmol/cell)	EtOH	Perifosine	% of control	p-value
In MCF-7 cancer cells
PCho (total, 31P)	30±5	15±1	51±5	0.005*
PCho (total, 1H)	27±5	15±4	55±4	0.003*
GPCho (total, 31P)	7±2	7±1	105±18	0.8
GPCho (total, 1H)	9±3	8±1	96±24	0.6
Choline (1H)	2±1	2±.2	78±31	0.3
tCholine (total, 1H)	38±5	25±5	65±5	0.004*
PtdCho (total, 31P)	29±5	23±3	79±6	0.08
PCho** (de novo, 13C)	6±2	3±1	51±12	0.025*
GPCho# (de novo, 13C)	1±0.2	2±0.3	223±62	0.003*
PtdCho (de novo, 13C)	9±0.4	10±1	104±14	0.249
Intracellular lactate (1H)	2.7±0.5	1.5±0.3	48±24	0.04*
Extracellular lactate (13C)	1348±427	640±276	55±33	0.04*
In MDA-MB-231 cancer cells
PCho(total, 1H)	38±9	19±8	50±8	.009*
GPCho(total, 1H)	11±2	10±2	90±19	0.7
tCholine(total, 1H)	52±12	29±9	55±11	0.03*
Intracellular lactate (1H)	3.3±0.9	1.8±0.6	55±18	0.03*

^*P<.05 (two tailed unpaired Student's t-test)

^**Concentrations determined from short-term 13C labeling

^#Concentrations determined from long-term ¹³C labeling