Figure 4.

(A) Confirmation of apoptosis mediated cell death in MCF-7 cells through observation of DNA laddering using DNA fragmentation assay on treated cells with EtOAc for 48 h followed by analysis of extracted DNA on 2% (w/v) agarose gel electrophoresis. No distinct fragmentations were observed in control lane indicating the absence of apoptosis as opposed to lanes 2, 3 and 4 (B) PARP degradation was observed in lane 200 μg/mL EtOAc. Equal amounts of cellular proteins were subjected to SDS-PAGE, and PARP degradation from its native form (116 kDa) to the cleaved form (89 kDa) was detected by Western blot analysis