Figure 2.

A: Dose-dependent growth inhibition of MCF-7 cells by solvent fractions of S. chloroleuca (0, 7.8, 15.6, 31.2, 62.5, 125 and 250 μg/mL) after 48 h. Viability was quantitated by MTS assay. The dose inducing IC50 against MCF-7 by MeOH, n-hexane, CH₂Cl₂, EtOAc, n-BuOH, and H₂O solvent fractions of S. chloroleuca were calculated 60.25, 28.06, 25.49, 63.14, >250, and >250 respectively.

B: The cytotoxic effect of total methanol extract of S. chloroleuca and n-hexane, CH₂Cl₂, EtOAc, n-BuOH, and H₂O fractions on normal lymphocyte proliferation isolated from peripheral blood. Paclitaxel (700 nM) was used as a positive control. Results are mean ± SEM (n = 3). *p <0.05, **p < 0.01 and ***p < 0.001 compared to control.

C: Morphological changes of MCF-7 cells after treatment with MeOH, n-hexane, and CH₂Cl₂ solvent fractions of S. chloroleuca (0, 7.8, 15.6, 31.2, 62.5, 125 and 250 μg/mL) after 48 h. Control cells remained untreated and received an equal volume of the solvent.