Figure 3.

A: Flow cytometry histograms of apoptosis assays by PI method in MCF-7 cells. Cells were treated with different concentration of CH₂Cl₂ solvent fractions of S. chloroleuca (0, 25, 50 and 100 μg/mL) for 48 h. Sub-G1 peak as an indicative of apoptotic cells, was induced in CH₂Cl₂ solvent fractions of S. chloroleuca treated but not in control cells. CH₂Cl₂ fraction-treated cells exhibited a sub-G1 peak in MCF-7 cells in a concentration dependent manner that indicates the involvement of an apoptotic process in CH₂Cl₂ fraction-induced cell death.

B: Internucleosomal fragmentation of CH₂Cl₂ fraction treated MCF-7 cells. Cells were treated with different concentration of CH₂Cl₂ fraction (lane 1, 2, 3, 4, 5 and 6 are 0, 12.5, 25, 50, 100 and 200 μg/mL respectively) for 48 h. After harvesting the cells, isolated DNA was analyzed by agarose gel electrophoresis.