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. Author manuscript; available in PMC: 2015 Jan 30.
Published in final edited form as: Cell Rep. 2014 Jan 16;6(2):254–263. doi: 10.1016/j.celrep.2013.12.034

Figure 4. DOCK7 Regulates ChC Cartridge/Bouton Development by Modulating ErbB4 Activity.

Figure 4

(A-D) Representative images of ChCs in layer II/III of somatosensory cortex from mice co-electroporated at E12.5 with plasmids expressing EGFP and empty control vector, ErbB4-E836K, Dock7#2 shRNA + vector, or Dock7#2 shRNA + ErbB4-E836K, and sacrificed at P28. Enlarged view of ChC cartridges with boutons is depicted on the right. Scale bars, 20 μm (left) and 5 μm (right).

(E-F) Quantification of bouton density (E) and size (F). Data are means ± SEM; 10-11 ChCs from 3 animals were analyzed for each condition, and for each cell 7-20 cartridges (E) and 90-162 boutons (F) were analyzed. ***p < 0.001, as compared with vector; oneway ANOVA, post hoc Tukey-Kramer test.

(G-J) Representative images of ChCs in layer II/III of somatosensory cortex from mice co-electroporated at E12.5 with plasmids expressing EGFP and scr#2 shRNA, ErbB4 shRNA, Flag-DOCK7 + scr#2 shRNA, or Flag-DOCK7 + ErbB4#2 shRNA, and sacrificed at P28. Enlarged view of ChC cartridges with boutons is depicted on the right. Scale bars, 20 μm (left) and 5 μm (right).

(K-L) Quantification of bouton density (K) and size (L). Data are means ± SEM; 9-17 ChCs from 3 animals were analyzed for each condition, and for each cell 7-17 cartridges (K) and 91-158 boutons (L) were analyzed. ***p < 0.001, as compared with scr#2; oneway ANOVA, post hoc Tukey-Kramer test.