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. 2013 Nov 4;23(4):380–392. doi: 10.1089/scd.2013.0314

FIG. 2.

FIG. 2.

Enzymatic removal of the NFC hydrogel. (A, B) Mitochondrial metabolic activity of WA07 and iPS(IMR90)-4 cells (A) and H9-GFP cells (B) as a function of the concentration of cellulase. Cells cultured on a standard Matrigel platform were treated with cellulase at various concentrations (μg cellulase/mg cellulose). The mitochondrial metabolic activity was determined by an AlamarBlue® assay, and the increase in fluorescence intensity after a 24-h treatment with cellulase is presented. Four (A) or six (B) biological samples for each condition were prepared. The results are expressed as mean±standard deviation (SD). (C) Removal of cellulose by 0, 50, 200, and 500 μg cellulase/mg cellulose is visualized by calcofluor white staining of cellulose, which is represented in blue. H9-GFP cells are seen in green. (D) A pluripotency marker OCT4 is expressed in H9-GFP cells that are cultured in 0.5 wt.% NFC hydrogel after treatment with cellulase at 50, 200, and 500 μg/mg cellulose. Scale bars=100 μm. **P<0.01 and ***P<0.001. Color images available online at www.liebertpub.com/scd