FIG. 3.

OCT4 expression in the 3D human pluripotent stem cell (hPSC) spheroids. The pluripotency marker OCT4 is expressed in WA07 cells that are cultured in 0.5% NFC hydrogel for 7 days [(A), 5 μm paraffin section], for 9 days (B), for 16 days after one subculture (C), and for 26 days after three subcultures [(D), 5 μm paraffin section]. OCT4 is also expressed in iPS(IMR90)-4 cells that are cultured in 0.5 wt.% NFC hydrogel for 9 days (E). Scale bars=200 μm (A) and 100 μm (B–E). Color images available online at www.liebertpub.com/scd