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. 2013 Nov 4;23(4):380–392. doi: 10.1089/scd.2013.0314

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 4. — Gene expression in hPSCs cultured in 3D and two-dimensional (2D) platforms. Real-time quantitative reverse transcription–polymerase chain reaction (RT-PCR) analyses of OCT4 (A) and NANOG (B) mRNA expression in WA07 cells cultured in the NFC hydrogel for 9 days and iPS(IMR90)-4 cells cultured in the hydrogel for 12 days and then transferred to 2D platforms [3D to M, 3D to laminin-511 (LN-511), 3D to LN-521, and 3D to vitronectin (VN)] as well as WA07 cells in 3D spheroids at days 7 (3D day 7) and 26 (3D day 26). Relative mRNA expression was normalized to the control gene RPLP0, and fold inductions were calculated with reference to the cells cultured on the standard Matrigel platform (M ctrl). n=3 biological samples. Error bars are SD. ***P<0.001.