FIG. 8.

In vivo differentiation of WA07 cells. (A–J) Hematoxylin and eosin staining of 6-week-old teratoma sections (5 μm) of WA07 cells that had previously been cultured in 0.5 wt.% NFC hydrogel for 26 days. (A) Cartilage (1), bone (2), epithelial tissue (3), and pigmented cells (4). (B) Neuronal rosettes (ectoderm). (C) Pigmented cells (ectoderm). (D) Blood vessels (indicated by asterisks, mesoderm). (E, F) Muscle (indicated by arrows, mesoderm). (G) Cartilage (mesoderm). (H–J) Endodermal epithelia (indicated by arrows). Scale bars=100 μm. (K) Real-time quantitative RT-PCR analyses of PAX6, CDX2, and BRACHYURY mRNA expression in 6-week-old teratoma formed from WA07 cells that had previously been cultured in 0.5 wt.% NFC hydrogel for 26 days. Relative mRNA expression was normalized to the control gene RPLP0, and fold inductions were calculated with reference to the cells cultured on the standard Matrigel platform (M ctrl). Color images available online at www.liebertpub.com/scd