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. 2014 Feb 10;55(2):824–831. doi: 10.1167/iovs.13-13755

Figure 1.

Figure 1

Generation of lentivector-delivered shRNA for specific knockdown of VEGF164 in Müller cells. HEK reporter cell lines expressed GFP-tagged VEGF120 or VEGF164 were transfected with RFP-expressed lentivector VEGF164.shRNA plasmids or empty vector without shRNA. (A) Flow cytometry (fluorescence activated cell sorting [FACS]) of GFP fluorescence. (B) Quantification of percent silencing of VEGF120 and VEGF164 by VEGF164 shRNAs from FACS analysis. (C) Real-time PCR of mRNA of VEGF120 and VEGF164 in rMC-1 infected without lentivirus (uninfected) or with lentivector-driven shRNA to luciferase (luc.shRNA), VEGFA (VEGFA.shRNA), or VEGF164 (VEGF164.shRNA). *P < 0.05 and **P < 0.01 versus luc.shRNA).