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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Gastroenterology. 2013 Oct 9;146(1):210–221.e13. doi: 10.1053/j.gastro.2013.09.060

Figure 5.

Figure 5

IL-10 is necessary for LL-IL-27’s therapeutic effect. CD4+CD45Rbhi T cells from C57BL/6 mice or IL-10−/− mice were transferred to Rag−/− mice. Following the onset of enterocolitis at 7.5 weeks, mice were gavaged with LL-IL-27 or LL-IL-10 for 14 days. Colons were harvested upon death (UT and LL-control) or the day after the last gavage (LL-IL-27). (A) Percent survival following T cell transfer (B) Representative H&E-stained sections of distal colons (left). Scale bar = 100 μm. Histopathological scores were determined for the distal colon (right) (C) Tissue homogenates were analyzed for IL-10 in mice gavaged with LL-IL-27 at the normal dose (ND) or at a 10-fold lower dose (LD), or LL-IL-10. Duo: duodenum, Jej: jejunum, Ile: Ileum, PC: proximal colon, DC: distal colon. (n=3–5, data representative of three independent experiments). Data represents mean ± s.e.m. *P ≤ .05, **P ≤ .01, determined by two-tailed Student’s t test.