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. 2013 Sep 27;23(4):406–420. doi: 10.1089/scd.2013.0412

FIG. 1.

FIG. 1.

Stage-specific dopaminergic differentiation protocol. (A) Schematic depiction of the differentiation protocol with the list of representative markers at the each stage. (B–E) Phase contrast (B) and immunocytochemistry images in pluripotent stem cells (PSCs) with antibodies against OCT4 (C), TRA1–60 (D), and SSEA4 (E). (F–I) Phase contrast (F) and immunocytochemistry images in neural stem cells (NSC) with antibodies against SOX1/Nestin (G), SOX2 (H), and PAX6 (I). (J–M) Phase contrast (J) and immunocytochemistry images in dopaminergic precursors (DA1) with antibodies against FOXA2/TH (K), LMX1A (L), and β-III-tubulin (M). (N–Q) Phase contrast (N) and immunocytochemistry images in mature dopaminergic population (DA2) for neuronal/dopaminergic markers β-III-tubulin/TH (O), astrocyte marker GFAP (P), and oligodendrocyte marker GALC (Q). Blue—DNA. Scale bar as marked.