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. 2013 Sep 27;23(4):406–420. doi: 10.1089/scd.2013.0412

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 3. — The effect of repression of sonic hedgehog (SHH) and WNT1 signaling pathways on dopaminergic differentiation. (A–C) qPCR analysis of SMO (A), WNT1 (B), and TH (C) gene expression following SMO (A), WNT1 (B), SMO, or WNT1 small interfering RNA (siRNA) treatment. (D–G) Representative TH (green) and β-III-tubulin (red) immunocytochemistry images following SMO (D), WNT1 (E), scrambled siRNA treatment (F), or in nontransfected cells (G). Scale bar as marked. (H, I) Quantification of TH-positive cells following SMO siRNA (H) or WNT1 siRNA (I) treatment.