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The pLVX-Puro-HS-GR vector was constructed by cloning HS-GR cassette into CMV driven lentiviral backbone (Clontech) between XhoI and XbaI restriction sites. H2B: histone protein sequence; 2A: self-cleavage peptide; SS: endoplasmatic reticulum signal peptide; GPI: C terminal GPI anchor sequence; pA: bovine growth hormone polyadenylation signal.
