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. 2014 Jan 23;2014:129048. doi: 10.1155/2014/129048

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Copyright © 2014 Sebastian Gehmert et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(a) Effect of conditioned medium on myoblasts' proliferation is shown as percentage after 48 h myostatin exposure. Proliferation of murine myoblast cultured in 0.75 μg/mL myostatin significantly decreased to 54.8% (±3.2%, **P < 0.001), whereas conditioned medium of ASCs significantly increased myoblasts' proliferation under 0.75 μg/mL myostatin incubation to 76.4% (±2.7%, **P < 0.001). This protective effect was abolished after neutralization of IGF-1 ligand-receptor interaction and proliferation significantly decreased to 65.8% (±2.9%, **P < 0.001). (b) Myoblasts exposed to 0.75 μg/mL myostatin showed a significant increase in apoptosis rate to 75.5% (±8.7%) but were reduced to 22.8% (±4.2%) due to paracrine factors of ASCs conditioned medium (**P < 0.001). The protective effect of ASCs conditioned medium was abolished after blocking IGF-1 and its receptor whereas apoptotic rate increased to 66.3% (±7.3%) (**P < 0.001). Thus, IGF-1 secreted by ASCs accounts for 82.5% of the antiapoptotic effect of the CM (N = 3, n = 3).