Table 1.

Thermodynamic data for different repeat bulge loop sizes:

Oligomer* Complex	Tm [°C]	ΔHcal [kcal mol−1]	ΔScal** [cal mol−1 K−1]	ΔCp [cal mol−1 K−1]	ΔGcal25°C [kcal mol−1]
CAG0·CTG0 duplex	79.1	173.4	492.3 ± 24	1100	21.8
CAG- loops
CAG2·CTG0	67.0	160.1	470.5	1260	16.4
CAG4·CTG0	63.7	164.3	487.7	1390	15.6
CAG6·CTG0	62.3	171.6	511.6	1540	15.7
CAG8·CTG0	61.3	192.2	574.6	1750	17.3
CTG-loops
CTG2·CAG0	67.1	157.4	462.7	380	18.5
CTG4·CAG0	63.1	162.3	482.7	890	16.4
CTG6·CAG0	61.9	185.5	553.6	1170	17.9
CTG8·CAG0	62.2	208.0	620.1	1570	19.7
T-loops***
T2·CTG0	65.2	155.2	458.2	1060	15.8
T4·CTG0	60.5	148.8	446.1	1330	13.2
T6·CTG0 ***	58.6	139.1	419.3	730	12.8
T8·CTG0	56.2	137.1	416.3	1230	11.1
				Ave 1090 ± 260

^*We estimate experimental errors to be T_m≈0.3°C, ΔH ≈ 2.5%, ΔS≈5% and ΔC_p ≈20%

^**Entropy values determined for pseudo-monomolecular process for Ct =50 µM, as bulge loops of different loop size show variable concentration dependence of Tm. To correct for bimolecular process a mixing term of 21.5 cal mol⁻¹ K⁻¹ needs to be added to the reported value.

^***These all T-bulge loops also form the basis of the DNA meter concept.^{97, 97}

^****Larger error for T6·CTG0 due to older commercially synthesized sample that was less well purified.