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. Author manuscript; available in PMC: 2014 Mar 10.
Published in final edited form as: J Control Release. 2012 Nov 12;166(2):124–129. doi: 10.1016/j.jconrel.2012.11.004

Figure 2. HB-EGF coacervate stimulates primary human keratinocyte migration without inhibiting proliferation in vitro.

Figure 2

(a) Representative images of scratch wounds on confluent keratinocyte monolayers after 24 h of culture in media supplemented with saline (Control), 100 ng ml−1 HB-EGF free or in the coacervate. Bars = 200 μm. (b) Quantification of percent closure of scratch wounds over time. Bars indicate means ± SD. *P<0.05; **P<0.01. (c) Quantification of cell number after 4d culture of keratinocytes in media supplemented with saline (Control), 1 ng ml−1 or 10 ng ml−1 HB-EGF free or in the coacervate. Bars indicate means ± SD. **P<0.01.