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In vitro mineralization of iChonInd and ATDC5. Following 21 days of in vitro differentiation, dark regions (white arrows) became visible in iChonInd (D) and ATDC5 (F) micromasses. No distinct dark regions were visible in iChonCon (A), iChonInd (C), and ATDC5 (E) cultured in BM or in iChonCon (B) cultured in differentiation medium.
