FIG. 4.

Toluidine Blue staining and CM-DiI labeling of iChon cells. Implants seeded with iChon^Con cells were able to form stable cartilage in vivo (A, B). No cartilage formation was detected in scaffolds containing iChon^Ind (C, D). CM-DiI labeling indicated the presence of donor cells in iChon^Con implants upon explantation (E). Quantification of Toluidine Blue staining revealed that implants seeded with iChon^Con contained 19.37%±0.87% cartilage, whereas only 3.56%±0.77% (p=2.6×10⁻¹⁴) positive staining was detected in iChon^Ind (F).