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Nmnat3 overexpression prevented TNF-induced axon loss. Light microscopic findings 2 weeks after (a) PBS injection, (b) 10 ng TNF injection, (c) 10 ng TNF injection+EGFP transfection, or (d) 10 ng TNF injection+EGFP–Nmnat3 transfection. Scale bar=10 μm (a–d). (e) Effect of Nmnat3 overexpression on axon numbers in the optic nerve. Each column represents mean±S.E.M.; n=4–9 per group. *P<0.05
