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. 2013 Oct 31;4(10):e893. doi: 10.1038/cddis.2013.425

Figure 3.

Figure 3

Light stimulation inhibited the proliferation of human glioma cells. (a) Cell cycle analysis of U87 cells in the control, light, eYFP+light, ChETA, and ChETA+light groups 24 h after light stimulation. (b) Green-fluorescent EDU-incorporated cells were observed in the control, light, eYFP+light, ChETA, and ChETA+light groups at 24 h after the light stimulation. Hoechst 33342-stained nuclei are shown in blue; bar=100 μm. (c) Quantification of the percentage of EdU-positive cells in the control, light, eYFP+light, ChETA, and ChETA+light groups, n=8. (d) Western blot analysis of β-actin, cyclin D, and cyclin E protein expression in the control, light, eYFP+light, ChETA, and ChETA+light groups at 24 h after the light stimulation. (e) Quantification of cyclin D and cyclin E protein expression in the control, light, eYFP+light, ChETA, and ChETA+light groups. n=3. **P<0.01; ***P<0.001