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. 2013 Oct 31;4(10):e893. doi: 10.1038/cddis.2013.425

Figure 7.

Figure 7

Light stimulation inhibited the proliferation and induced the apoptosis of human glioma cells in vivo. (a) Immunostaining of nestin in the control, light, eYFP+light, ChETA, and ChETA+light groups. (b) EdU-positive cells (green) were observed in the control, light, eYFP+light, ChETA, and ChETA+light groups. Hoechst 33342-stained nuclei are shown in blue. (c) Immunostaining of cyclin D in the control, light, eYFP+light, ChETA, and ChETA+light groups. (d) TUNEL staining of apoptotic U87 cells in the control, light, eYFP+light, ChETA, and ChETA+light groups; Hoechst 33342-stained nuclei are shown in blue. (e) Quantification of the TUNEL-positive cells in the control, light, eYFP+light, ChETA, and ChETA+light groups. (f) Schematic drawing for the mechanism of the light-induced regulation of glioma cell proliferation and apoptosis. Bar=50 μm (a–d). ***P<0.001. The experiments were repeated for three times with similar results