Figure 8.

mTORC1/2 kinase inhibition reverses CPT-induced cell death in ccRCC. (a) RCC4/VHL and 786-O/VHL cells were incubated with (open bars) or without (solid bars) 400 nM pp242 for 1 h before addition of increasing concentrations of CPT as indicated for 24 or 48 h. Cell viability was determined by reduction of MTT at A 595 nm. Mean±S.E. of triplicate values is shown. (b) RCC4/VHL and 786-O/VHL cells were incubated with 400 nM pp242 or 500 μM DFX for 1 h before addition of 2 μM CPT as indicated for 48 h. Whole-cell lysates were assayed for cleaved caspases 3, 7 and 9 and cleaved PARP. Actin was used as a loading control