Figure 1.

Src42A is required for Ras-lgl oncogenic cooperation and loss of scrib-induced invasion. (a–c) GFP-labeled clones of cells with indicated genotypes are created in the developing eye–antennal discs. Ras^V12/lgl^−/−-induced tumor growth (a) and invasion of the VNC (a′) were dramatically suppressed by expressing Puc (b–b′). The invasion behavior was also strongly suppressed by expressing an src42A RNAi (c′), whereas the tumor overgrowth was not significantly changed (c). Scale bars, 200 μm in a–c and 100 μm in a′–c′. (d–f) Fluorescence micrographs of wing discs are shown. Compared with the control (d–d′′), loss of scrib-induced cell invasion and MMP1 expression (e–e′′) was suppressed by the expression of an src42A RNAi (f–f′′). Scale bars, 20 μm