Figure 5.

Src42A expression activates JNK in vivo. (a–b) Fluorescence micrographs of wing discs are shown. Compared with the controls (a), Src42A expression induced strong JNK phosphorylation (b). Scale bars, 100 μm. (c–f) X-Gal staining of the puc-LacZ reporter gene in the developing wing (c–d) and eye (e–f) are shown. Ectopic Src42A expression under the ptc promoter strongly upregulated puc expression along the A/P boundary (d). The weak endogenous puc expression posterior to the morphogenetic furrow of eye disc (e) was significantly enhanced by expressing a wild-type Src42A (f)