Figure 3.

(a) TSA inhibited LECs proliferation through suppressing the PI3K/Akt, p38MAPK and ERK1/2 signaling pathways. SRA01/04 and HLEB3 cell lines were treated with TSA at various concentrations (0.1, 0.2, 0.4 and 0.8 μM) for 60 min, then the protein expression levels of PI3K, p-Akt, Akt, p-p38MAPK, p38MAPK, p-ERK1/2 and ERK1/2 were detected by western blot analysis. (b) TSA increased the levels of acetylated histones H3 and H4. The expression of acetylated histones H3 (Lys 9 and 14) and H4 (Lys8) were detected after 12 h treatment. β-Actin, loading control. All experiments were repeated three times with similar results