Figure 4.

TSA prevents TGFβ2-induced EMT in LECs. Cells were cultured in the absence or presence of TGFβ2 with TSA or DMSO for 24 h. (a) The mRNA expression levels of FN, Col I, Col IV and N-cadherin in SRA01/04 and HLEB3 cell lines were determined by real-time quantitative PCR. Gene expression levels were normalized to the glyceraldehyde 3-phosphate dehydrogenase control. *P<0.05 versus TGFβ2 treated with DMSO group. (b) The protein expression levels of FN, Col I, Col IV and N-cadherin in SRA01/04 and HLEB3 cell lines were detected by western blot analysis. β-Actin, loading control. All experiments were repeated three times with similar results