Figure 7.

TSA abrogates TGFβ2-induced EMT via downregulating the Notch pathway. (a) The mRNA expression levels of Jagged-1, Notch-1, Notch-2, Hes-1 and Hey-1 were detected by real-time PCR in SRA01/04 cell line treated with TGFβ2 in the presence of TSA (0.2 μM) or DMSO for 24 h. (b) The mRNA expression levels of Jagged-1, Notch-1, Notch-2, Hes-1 and Hey-1 in HLEB3 cell line were also detected by real-time PCR. Gene levels were normalized to control glyceraldehyde 3-phosphate dehydrogenase. *P<0.05 versus TGFβ2 treated with DMSO group. (c) Representative Jagged-1, Notch-1, Notch-2 and N1ICD immunoblot from SRA01/04 and HLEB3 cell lines treated with TGFβ2 in the presence of TSA (0.1, 0.2 0.4 and 0.8 μM) or DMSO for 24 h. All experiments were repeated three times with similar results