Figure 1.

LY30 restores sensitivity to TRAIL-mediated cell death. (a) Crystal violet viability assay of HeLa cells treated with treated with TRAIL alone (0–250 ng/ml) for 24, 48 or 72 h. (b) CI of LY30 and TRAIL calculated with the Chou-Talalay method. (c) Crystal violet viability assay of HeLa cells treated with TRAIL (0, 10 and 20 ng/ml) for 16 h with or without 1 h preincubation with various doses of LY30 (0–6.125 μM). (d) Crystal violet viability assay of HeLa cells treated with 25 μM of LY30 and 20 ng/ml of TRAIL for various durations. (e) Trypan blue exclusion assay for HeLa cells treated with 20 ng/ml of TRAIL for 16 h with or without preincubation with 25 μM LY30. (f) Colony-forming assay for HeLa cells treated with 20 ng/ml of TRAIL for 6 h with or without preincubation with 25 μM LY30 before re-seeding. Colony count and colony size measurements are also provided. Data shown are the mean±S.D. of three independent experiments. NT: not treated, LY: LY30, T: TRAIL, LYT: LY30+TRAIL