Figure 2.

Effect of Hsp27 expression modulation. (a) Western blot analysis of non transfected Hela cells, cells transiently overexpressing Hsp27 or cells where Hsp27 has been knocked down. The indicated durations represent the time after transfection. (b) Crystal violet viability assay of HeLa cells after 16 h treatment with TRAIL (0–250 ng/ml) in the presence or absence of 25 μM LY30 in non transfected cells, (c) in cells transiently overexpressing Hsp27 or (d) in cells where Hsp27 has been knocked down. (e) Crystal violet viability assay of HeLa cells transfected with negative siRNA or specific for Hsp27 and treated with TRAIL (0–250 ng/ml) for 24, 48 and 72 h. (f) Colony formation assay in cells transfected with negative siRNA or specific for Hsp27 and treated with 20 ng/ml TRAIL. Colony count and colony size are provided