Figure 3.

Changes in Hsp27 localization but not expression upon exposure to LY30. (a) Western blot analyses of Hsp27, Hsp70 and Hsp90 after 20 h of treatment with LY30. (b) Total mRNA levels of Hsp27 in HeLa cells after treatment for different durations. (c) Western blot analysis of Hsp27 levels after 16 h of treatment in the presence or absence of 10 μM lactacystin (2 h preincubation). (d) Western blot analysis of Hsp27 in the pellet and supernatant fractions of cleared (centrifuged) cell lysates after 6 or 16 h of treatment, (e) in whole-cell lysates after 4, 8 or 20 h of treatment or (f) in the pellet and supernatant fractions of sonicated cell lysates after 16 h of treatment