Figure 6.

Effect of LY30 on Hsp27 oligomers size distribution. (a) HeLa cells were treated with 25 μM LY30 for 1 h in the presence or absence of 10 μM SB203580 or 10 μM okadaic acid (OA) (2 h preincubation) or (b) treated for 6 h with 20 ng/ml TRAIL with or without preincubation with 25 μM LY30. The cell extracts were fractionated by centrifugation on glycerol gradient. Each fraction was slot-blotted and Hsp27 was detected by immunoblotting using anti-HSP27. Immunoblots were then analyzed by densitometry. (c) Glutaraldehyde cross-linking of cell lysates from cells treated for 1 h with 20 ng/ml TRAIL with or without preincubation with 25 μM LY30