Figure 5.

(a) Ex vivo analyses of lymphocytes isolated from patients with CLL. Flow cytometry analysis of surface expression level of CD19 in lymphocytes freshly isolated from a representative HD among six or from two pathological subjects among six (Pt1 and Pt2) affected by B leukemia. Numbers represent the percentage of CD19-positive cells. Results obtained in a representative experiment are shown. (b) Surface expression of TRAIL receptors. Flow cytometry analysis of surface expression level of DR4 and DR5 in lymphocytes freshly isolated from a healthy donor or from two pathological subjects affected by B leukemia. Numbers represent the median fluorescence intensity. Results obtained in a representative experiment are shown. (c) Apoptosis induction by sTRAIL, mTRAIL and agonist antibodies to DR4 and DR5. Biparametric flow cytometry analysis of apoptosis induced by sTRAIL or by mTRAIL, that is, coculturing CD34⁺-armed cells or CD34⁺-mock with target cells (1 : 1 ratio). Numbers represent the percentage of apoptotic cells either annexin V/Trypan blue double-positive or annexin V single-positive. Results obtained in a representative experiment are shown. Analyses shown in b and c were restricted to CD19-positive cells