Figure 6.

Ex vivo analyses of lymphocytes isolated from patients with CLL. Flow cytometry analysis of lymphocytes freshly isolated from a representative HD among six or from two pathological subjects among six affected by B leukemia (Pt1 and Pt2) triggered for apoptosis. Bar graphs showing the amount of apoptosis induced by (a) sTRAIL, (b) anti-DR4 or anti-DR5 agonist antibodies or (c) coculturing CD34⁺-armed cells with target cells (1 : 1 ratio) in control cells and in cells pretreated with perifosine before apoptotic triggering. Note that representative Pt1 was susceptible to all the pro-apoptotic stimuli mentioned above. Mean±S.D. of the percentages of annexin V-positive cells obtained in four different experiments is reported. * indicates P<0.01; ° indicates P<0.05 versus sTRAIL sample (a), versus anti-DR4 agonist Ab sample (b) or versus CD34⁺-armed sample (c)