Figure 1.

Survival and functional activity of HL-1 cardiac cells during 48 h of starvation. HL-1 cells were treated with UA-8 (1 μM) in the presence or absence of 14,15-EEZE (10 μM) in amino acid-free and serum-free starvation buffer. (a) Cell viability was assessed by Trypan blue exclusion. (b) Total mitochondrial activity was measured by MTT assay. (c) Alterations in colony formation ability of HL-1 cells starved for 24 h with and without UA-8. (d) Effect of UA-8 on contractility of HL-1 cells starved for 24 h. (e) Changes in caspase-3 activity of HL-1 cells starved with and without UA-8. (f) Changes in total proteasome activity of HL-1 cells starved with and without UA-8. (g) Effect of UA-8 on total antioxidant capacity of HL-1 cells starved for 24 h. Values are represented as mean±S.E.M., N=3. Significance was set at P<0.05, *significantly different from control nonstarvation or statistically not different (ND), ^#significantly different from UA-8