Figure 2.

Effect of UA-8 treatment on starvation-induced cellular stress responses in NCMs. NCMs were treated with UA-8 (1 μM) in the presence or absence of 14,15-EEZE (10 μM) in amino acid-free and serum-free starvation buffer for 24 h. Starvation induced activation of caspase-3 (a) and proteasome activity (b) in NCMs. (c) UA-8 potentiated the beating rate of nonstarved (NS) NCMs and prevented starvation-induced decline of the beating rate in starved (STV) NCMs. (d) Alterations in total antioxidant capacity of NCMs exposed to starvation for 24 h with and without UA-8. Cotreatment with 14,15-EEZE antagonized the effect of UA-8. Values are represented as mean±S.E.M., N=3. Significance was set at P<0.05, *significantly different from control nonstarvation or statistically not different (ND), ^#significantly different from UA-8