Table 1.
Characterization of mAb CZP-315.D9 with the SBA Clonotyping-HRP System. Numbers are the optical density (OD) values read at 450 nm. Only OD values above 0.300 were considered positive. RPMI medium was used as a negative control.
Ig (H+L) | IgM | IgA | IgG1 | IgG2a | IgG2b | IgG3 | Kappa | Lambda | |
---|---|---|---|---|---|---|---|---|---|
Anti-TcCruzipain serum | 1.207 | 0.852 | 0.585 | 1.24 | 0.816 | 0.996 | 0.19 | 1.125 | 0.33 |
Hybridoma 315 | 0.99 | 0.085 | 0.077 | 1.259 | 0.512 | 0.078 | 0.069 | 0.362 | 0.058 |
mAb CZP-315.D9 | 0.788 | 0.055 | 0.056 | 1.105 | 0.085 | 0.164 | 0.065 | 0.459 | 0.133 |
Medium (RPMI) | 0.059 | 0.056 | 0.049 | 0.064 | 0.068 | 0.061 | 0.072 | 0.108 | 0.059 |