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GrB/VEGF121 internalization into PAE/VEGFR-2 was driven by VEGF121. Cells were (A) untreated or (B) treated with 20 nM GrB/VEGF121 for 24 h, followed by detection of GrB. Nuclei were stained with propidium iodide. Only nuclei were visible in untreated PAE/VEGFR-2 cells whereas fluorescent GrB staining was observed in the cytoplasm of GrB/VEGF121-treated cells.
