Figure 3.
SWNHs inhibited mitotic entry of liver cells.
Notes: L02 cells (3×105) and HepG2 cells (3×105) were seeded onto 60 mm noncoated and SWNH-coated dishes, respectively, and cultured for 48 hours. To assess how the SWNHs affected cellular mitosis of L02 (A) and HepG2 (B) cells, we incorporated BrdU into the control, and accumulations of both mitotic entry of L02 (C) and HepG2 (D) were counted. (A) The mitotic entry of L02 cells treated with SWNHs decreased significantly after culture for 24 hours and 36 hours (P<0.05), and especially after 48 hours (P<0.01). (B) The mitotic entry of HepG2 cells treated with SWNHs decreased significantly after culture for 12 hours (P<0.05), and especially after 24 hours, 36 hours, and 48 hours (P<0.01). (C) The cumulative mitotic entry of L02 cells decreased significantly from 14 hours after culture with SWNHs 10 and from 12 hours after culture with SWNHs20, SWNHs30, and SWNHs40 (P<0.01). (D) The cumulative mitotic entry of HepG2 cells decreased significantly from 12 hours after culture with SWNHs10 (P<0.05), and especially from 12 hours after culture with SWNHs20, SWNHs30, and SWNHs40 (P<0.01). All data are represented as mean ± SEM. *P<0.05; **P<0.01.
Abbreviations: SWNH, single-walled carbon nanohorn; L02, normal liver cell line; HepG2, human hepatoma cell line; BrdU, bromodeoxyuridine; SWNHs10, 0.21 μg of single-walled carbon nanohorns per cm2, or 6 μg per 60 mm dish; SWNHs20, 0.42 μg of single-walled carbon nanohorns per cm2, or 12 μg per 60 mm dish; SWNHs30, 0.64 μg of single-walled carbon nanohorns per cm2, or 18 μg per 60 mm dish; SWNHs40, 0.85 μg of single-walled carbon nanohorns per cm2, or 24 μg per 60 mm dish; SEM, standard error of the mean.