Figure 6.
SWNHs promoted cell apoptosis of liver cells, and apoptosis involves key factors in vivo.
Notes: L02 cells l (3×105) and HepG2 cells (3×105) were seeded onto 60 mm noncoated and SWNH-coated dishes, respectively, and cultured for 48 hours. Then the effect of SWNHs on L02 (A) and HepG2 (B) cell apoptosis distribution was determined by flow cytometry. The expression levels of SIRT1 and those of activation cleavage of p53, caspase-3 and caspase-7 L02 (C) and HepG2 (D) were determined by Western Blotting. (A) The apoptotic L02 cells treated with SWNHs increased significantly at 48 hours after culture with SWNHs10 (P<0.05), and especially with SWNHs20, SWNHs30, and SWNHs40 (P<0.01) (B) The apoptotic HepG2 cells treated with SWNHs increased significantly at 48 hours after culture with SWNHs10 (P<0.05), and especially with SWNHs20, SWNHs30, and SWNHs40 (P<0.01). All data are represented as mean ± SEM. *P<0.05; **P<0.01.
Abbreviations: SWNH, single-walled carbon nanohorn; L02, normal liver cell line; SWNHs10, 0.21 μg of single-walled carbon nanohorns per cm2, or 6 μg per 60 mm dish; SWNHs20, 0.42 μg of single-walled carbon nanohorns per cm2, or 12 μg per 60 mm dish; SWNHs30, 0.64 μg of single-walled carbon nanohorns per cm2, or 18 μg per 60 mm dish; SWNHs40, 0.85 μg of single-walled carbon nanohorns per cm2, or 24 μg per 60 mm dish; HepG2, human hepatoma cell line; SEM, standard error of the mean; SIRT1, silent information regulator 1.